PREreviews of “Medicago truncatulaZinc-Iron Permease6 provides zinc to rhizobia-infected nodule cells”

Skip to preprint details Skip to PREreviews

Medicago truncatulaZinc-Iron Permease6 provides zinc to rhizobia-infected nodule cells

by Isidro Abreu, Ángela Saéz, Rosario Castro-Rodríguez, Viviana Escudero, Benjamín Rodríguez-Haas, Marta Senovilla, Camille Larue, Daniel Grolimund, Manuel Tejada-Jiménez, Juan Imperial, and Manuel González-Guerrero

Posted: January 24, 2017
Server: bioRxiv
DOI: 10.1101/102426

Abstract

Zinc is a micronutrient required for symbiotic nitrogen fixation. It has been proposed that in model legumeMedicago truncatula, zinc is delivered in a similar fashion as iron,i.e.by the root vasculature into the nodule and released in the infection/differentiation zone. There, zinc transporters must introduce this element into rhizobia-infected cells to metallate the apoproteins that use zinc as a cofactor.MtZIP6(Medtr4g083570) is aM. truncatulaZinc-Iron Permease (ZIP) that is expressed only in roots and nodules, with the highest expression levels in the infection/differentiation zone. Immunolocalization studies indicate that it is located in the plasma membrane of rhizobia-infected cells in the nodule. Down-regulatingMtZIP6expression levels with RNAi does not result in any strong phenotype when plants are being watered with mineral nitrogen. However, these silenced plants displayed severe growth defects when they depended on nitrogen fixed by their nodules, as a consequence of the loss of 80% of their nitrogenase activity. The reduction of this activity was not the result of iron not reaching the nodule, but an indirect effect of zinc being retained in the infection/differentiation zone and not reaching the cytosol of rhizobia-infected cells. These data are consistent with a model in which MtZIP6 would be responsible for zinc uptake by rhizobia-infected nodule cells in the infection/differentiation zone.

Read the preprint

1 PREreview

Write a PREreview Request a PREreview

PREreview by Elsbeth Walker et al.

Authored by Elsbeth Walker, Ahmed Ali, Rakesh Krishna Kumar, Miriam Hernandez Romero, Maura Zimmermann, Gurpal Singh, Rebecca Brennan, David Chan-Rodriguez, Stavroula Fili, Erin Patterson, and Ahmet Bakirbas

Review of Medicago truncatula Zinc-Iron Permease6 provides zinc to rhizobia-infected nodule cells DOI: 10.1101/102426 (BioRxiv), 10.1111/pce.13035 (Plant Cell & Environment)
Medicago truncatula Zinc-Iron Permease6 provides zinc to rhizobia-infected nodule cells
[Isidro Abreu, Angela Saez, Rosario Ca…
Read the PREreview by Elsbeth Walker et al.

PREreviews of Medicago truncatulaZinc-Iron Permease6 provides zinc to rhizobia-infected nodule cells

1 PREreview

Review of Medicago truncatula Zinc-Iron Permease6 provides zinc to rhizobia-infected nodule cells DOI: 10.1101/102426 (BioRxiv), 10.1111/pce.13035 (Plant Cell & Environment)

Medicago truncatula Zinc-Iron Permease6 provides zinc to rhizobia-infected nodule cells